Staining:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
Avidin-Biotin Assay:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
Blocking Assay:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
Protease Inhibitor:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
Western Blot:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
Isolation:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
Acid Assay:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
Control:Article Title: Bacterial extracellular vesicle ssRNA prevents colorectal cancer progression via Piezo1.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Piezo1 antibody Proteintech Cat# 15939-1-AP; RRID: AB_2231460 Normal rabbit immunoglobulin Dako Cat# X0936; RRID: AB_2940930 Biotin-conjugated anti-rabbit IgG Vector Cat# BA-1000; RRID: AB_2313606 anti-Piezo1 antibody Alomone Labs Cat# APC-087; RRID: AB_2756743 Mouse anti-Zfp281 antibody Abcam Cat# ab101318; RRID: AB_11157929 PIEZO1 Antibody Novus Biologicals Cat# NBP1-78446; RRID: AB_11020328 Anti-ZNF281 Polyclonal Thermo Fisher Scientific Cat# PA-116145: RRID: AB_2900779 Anti-LGR5 antibody GeneTex Cat# GTX130204; RRID: AB_2886205 Rabbit IgG Control Antibody VectorLabs Cat# I-1000; RRID: AB_2336355 Anti-NRF2 (D1Z9C) antibody Cell Signaling Cat#12721; RRID: AB_2715528 Mouse anti-b-actin pAb-HRP-DirecT MBL Cat# PM053-7; RRID: AB_10697035 HRP-conjugated anti-rabbit IgG Promega Cat# W4018 HRP-conjugated secondary antibody R&D Cat# HAF008; RRID: AB_357235 Medium and serum High-glucose Dulbecco’s modified Eagles’s medium with sodium pyruvate Fujifilm Cat# 043-30085 Fetal bovine serum Biowest Cat# S1810-500 Opti-MEM Gibco Cat# 31985062 Chemicals, peptides, and recombinant proteins Antibiotic-Antimycotic Gibco Cat# 15240062 GlutaMax Supplement Gibco Cat# 35050061 Trypsin-EDTA Gibco Cat# 25300054 CELL BANKER 1 Nippon Zenyaku Kougyo Cat# CB011 Collagenase type I Sigma Cat# SCR103 Ionomycin Fujifilm Cat# 095-05831 GsMTx4 Peptide Institute Cat# 4393-s Lithocholic acid (LCA) Sigma Cat# L6250 Deoxycholic acid (DCA) Sigma Cat# D2510 Chenodeoxycholic acid (CDCA) Sigma Cat# C9377 β-Muricholic acid (β-MCA) Cayman Chemical Cat# 20287 Cholic acid (CA) Wako Cat# 032-03042 Hyodeoxycholic acid (HDCA) Tokyo Chemical Industry Cat# H0535 Ursodeoxycholic acid (UDCA) Tokyo Chemical Industry Cat# U0030 DMSO Nacalai Tesque Cat# 13445-74 ML385 Selleck Cat# S8790 Doxycycline Takara Cat#631311 Tris-EDTA buffer solution Sigma Cat# 93302 Ampicillin Sigma Cat# A5354 Vancomycin Sigma Cat# V1130 Neomycin Sigma Cat# N1876 Metronidazole Sigma Cat# M3761 Isopropanol Nacalai Cat# 29113-95 Ursodeoxycholic acid-d4 Cayman Chemical Cat# 0655299-9 Acetic acid Fujifilm Wako Pure Chemicals Cat# AWL2657 (Continued on next page) Cell Reports 45, 116737, January 27, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Methanol Kanto Chemical Cat# 510Y1195 Acetonitrile Kanto Chemical Cat# 510K1752 Azoxymethane Fujifilm Wako Pure Chemicals Cat# 011-20171 Sodium dextran sulfate MW36000-50000 MP Biomedicals Cat# 160110 G-Nox Genostaff Cat# GN-04 Proteinase K Fujifilm Cat# 161-28701 G-wash Genostaff Cat# GW-01 G-Hybo-L Genostaff Cat# RPD-02 G-block Genostaff Cat# GB-01 Anti-DIG AP conjugate Roche Cat# 11093274910 NBT/BCIP solution Sigma Cat# 72091 Kernechtrot stain solution Muto Cat# 40872 G-mount Genostaff Cat# GM-01 Malinol Muto Cat# 20093 Tris-EDTA buffer Genostaff Cat# ARSE9-01 Avidin/biotin blocking kit Vector Cat# SP-2001 Peroxidase-conjugated streptavidin Nichirei Cat# 326062 Mayer’s hematoxylin Muto Cat# 30142 TRIzol Thermo Fisher Scientific Cat# 15596026 KOD FX neo DNA polymerase Toyobo Cat# KFX-201 KOD One DNA polymerase Toyobo Cat# KMM101 RNaseA Thermo Fisher Scientific Cat# 12091021 Matrigel Corning Cat# 356234 Polyethylenimine Max Polysciences Cat# 24765 Puromycin Invivogen Cat# ant-pr-1 KOD FX DNA polymerase Toyobo Cat# KFX-101 Complete protease inhibitor mixture tablet Roche Cat# 11836170001 Pierce ECL western blotting substrate Thermo Fisher Scientific Cat# 32106 Lipofectamine 3000 Thermo Fisher Scientific Cat# L300000088 Fluo-8 a.m. ATT Bioquest Cat# 21080 Yoda1 Sigma Cat# SML1558 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX reagent Thermo Fisher Scientific Cat# 13778100 Critical commercial assays ssRNA40 InvivoGen Cat# tlrl-lrna40 NucleoSpin TriPrep kit Macherey-nagal Cat# NC1205208 Exo Quick-TC Exosome Precipitation Solution System Biosciences Cat# EXOTC50A1 Every EV RNA Isolation Kit System Biosciences Cat# Every100B-1 Total Bile Acid Assay Kit Cell Biolabs Cat# STA-631 ReverTra Ace Toyobo Cat# FSQ201 TaqMan ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 NEB Stable Competent E. coli NEB Cat# C3040I In-Fusion Snap Assembly Takara Bio Cat# 638947 BigDye Terminator v1.1 Thermo Fisher Scientific Cat# 4337452 ExoSAP-IT Express PCR Cleanup Reagents Thermo Fisher Scientific Cat# 75001 Dynabeads Protein G Invitrogen Cat# 10003D CytoSelect BrdU Cell Proliferation ELISA Kit Cell Biolabs Cat# CBA-251 (Continued on next page) 16 Cell Reports 45, 116737, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MTT Cell Proliferation Assay Kit Cayman Cat# 10009365 RNeasy Mini Kit Qiagen Cat# 74104 Mouse CD9 antigen ELISA kit EIAab Cat# E15351m Mouse CD63 antigen ELISA kit EIAab Cat# E9307m Mouse CD81 antigen ELISA kit EIAab Cat# E9096m General Lipopolysaccharide ELISA kit EIAab Cat# E1526Ge LTA ELISA Kit Cell Biolabs Cat# AKR-5153 MagCapture Exosome Isolation Kit PS Fujifilm Cat# 293-77601 β-catenin cell-based enzyme-linked immunosorbent assay (ELISA) Kit LSBio Cat# LS-F1859 TCF/LEF Reporter Kit BPS Biosciences Cat# 60500 In-Fusion Snap Assembly Master Mix Takara Bio Cat# 638947 Ligation-Convenience kit Nippon Gene Cat# 315-05963 Polyethylenimine Max Polyscience Cat# 24765 Deposited data Microarray data of the CMT-93 Piezo1 − /− cell line Gene Expression Omnibus (GEO) (https:// www.ncbi.nlm.nih.gov/geo/) Accession number: GSE264013 Experimental models: cell lines CMT-93 cell line ATCC Cat# CCL-223 Franks & Hemmings 40 HEK293T cell line Provided by D. Julius N/A HEK293 Piezo1 − /− cell line Provided by A. Patapoutian Dubin et al. 92 CMT-93 Piezo1 − /− cell line Present study N/A CMT-93 Zfp281 − /− cell line Present study N/A CMT-93 Piezo1 − /− Zfp281 − /− cell line Present study N/A Experimental models: organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Germ free mouse Japan CLEA Strain ID: Mouse C57BL/6 [GF] Mouse: Piezo1 tm1a(KOMP)Wtsi KOMP repository Strain ID: Piezo1 tm1a Mouse: FLPo-expressing transgenic mice Provided by M. Ikawa Yamazaki et al. 93 Mouse: Villin-Cre Provided by S. Akira el Marjou et al. 94 Oligonucleotides Genotyping DNA primers (Table S2) Present study N/A Piezo1 (Accession No: NM_001037298.1; sequence length: 8216 bp; GC content: 59.04%) In situ hybridization probe: sequence position 902–1579 (678 bp); GC content: 58.06% Present study N/A TaqMan probes (Table S2) Life Technologies N/A Primers used for PCR and sequencing to confirm genome editing (Table S2) Present study N/A Lgr5 promoter ChIP primers (Table S2) Present Cheasley et al. 95 Stealth siRNA for mouse Lgr5; CCCUUGCUAGGAGGCAGUAAGUACA.
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